Interruption-deficient mutants of bacteriophage T5. II. Properties of a mutant lacking a specific interruption
نویسندگان
چکیده
منابع مشابه
Interruption-deficient mutants of bacteriophage T5: analysis of single-site mutants.
The properties of viable mutants of bacteriophage T5 that lack, singly, each of the four major sites at which single-chain interruptions normally occur in T5 DNA are described. The mutations responsible for loss of each interruption were mapped by analysis with HhaI, a restriction endonuclease with a cleavage site (pGCGC) that occurs at the 5' termini of the major interruptions (B. P. Nichols a...
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Nonsense mutations in structural genes appear to abort polypeptide chain synthesis at the amino acid site governed by the mutant codon. The two presently recognized types of nonsense mutant, amber and ochre, have been assigned the codons UAG and UAA, respectively. Certain bacteria harbor one or more genetic suppressors that counteract the effect of appropriate nonsense mutations in their own st...
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Bacteriophage T5 induces a deoxyuridine B’-triphosphate nucleotidohydrolase (dUTPase) activity during infection of Escherichiu coli. A T5 mutant (T5 dut) unable to induce this dUTPase activity has been isolated. Although this mutant is viable, the E. coli dUTPase activity is not sufficiently active to exclude uracil from the progeny DNA and about 3% of the thymine is replaced by uracil. When th...
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5, one of the classical T phages of Escherichia coli, has been the subject of many studies, but its genetic analysis has been sporadic. Beginning with heatstable ( s t ) mutants (ADAMS and LARK 1950; ADAMS 1953), there have been reports of mutants with altered host range (LANNI and LANNI 1956), serological behavior (LANNI and LANNI 1957), lysis-inhibiting properties (U. T. LANNI 1958), and buoy...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1979
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.29.2.726-734.1979